Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: LSD1 modulates stress-evoked transcription of immediate early genes and emotional behavior

doi: 10.1073/pnas.1511974113

Figure Lengend Snippet: Western blot analysis showing that the additional 57-kDa SRF-related band is present only in hippocampal protein extracts and not in a nonneuronal cell line (HeLa). Total protein lysates from HeLa cells (lane1) and mouse hippocampus (lane2) were separated on SDS/PAGE and immunodecorated with anti-SRF antibody and anti-GAPDH antibody.

Article Snippet: The following antibodies were used for Western blot or immunoprecipitation experiments: anti-LSD1 (C69G12; Cell Signaling Technology); anti-SRF (D71A9; Cell Signaling Technology); anti-CoREST (07-455; Merck Millipore); anti-HDAC2 (ab7029; Abcam); anti-GAPDH (NB300-320; Novus Biological); anti-α/β tubulin (2148; Cell Signaling Technology); and anti-HA (sc-7392; Santa Cruz Biotechnology).

Techniques: Western Blot, SDS Page

Journal: Yonsei Medical Journal

Article Title: Changes in Inward Rectifier K + Channels in Hepatic Stellate Cells During Primary Culture

doi: 10.3349/ymj.2008.49.3.459

Figure Lengend Snippet: Relative inward rectifier K + channel and SUR gene expression in HSC. (A) The inward rectifier K + channel α-subunit gene expression in HSC was measured using real-time RT-PCR. The bar graphs show the relative gene expression for each inward rectifier K + channel subfamily (K ir 1.1, K ir 2.1 - K ir 2.4, K ir 3.1 - K ir 3.4, K ir 4.1 - K ir 4.2, K ir 5.1, K ir 6.1 - K ir 6.2, and K ir 7.1). (B) SUR gene expression was measured. The bar graphs show the relative gene expression for SUR1, SUR2A, and SUR2B. The HSC were used at 1 day, 1 week, 2 weeks, and 3 weeks of culture. The expression levels were normalized to GAPDH and calibrated by K ir 2.1 expression in the HSC cultured for 1 day. The data are shown as the mean ± SEM (n = 3). (C) K ir 2.1 and K ir 6.1 protein expression were measured by Western blotting. There was a band (55 kD) in the anti-K ir 2.1 membrane (Upper) as well as the anti-K ir 6.1 membrane (Lower). Both membranes were re-probed with anti-GAPDH antibody as shown. The data is representative of three independent experiments.

Article Snippet: The anti-K ir 2.1 and anti-K ir 6.1 antibodies were obtained from Alomone Labs (Jerusalem, Israel), the anti-GAPDH antibody was acquired from Novus Biologicals (Littleton, CO, USA), and Western Lightning was supplied by PerkinElmer Life Sciences (Gaithersburg, MD, USA).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Cell Culture, Western Blot, Membrane